Predicting failure rate of PCR in large genomes
نویسندگان
چکیده
منابع مشابه
Predicting failure rate of PCR in large genomes
We have developed statistical models for estimating the failure rate of polymerase chain reaction (PCR) primers using 236 primer sequence-related factors. The model involved 1314 primer pairs and is based on more than 80 000 PCR experiments. We found that the most important factor in determining PCR failure is the number of predicted primer-binding sites in the genomic DNA. We also compared dif...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2008
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gkn290